这是一个用于流式细胞术或荧光显微镜的荧光色素的一些特性的表。Yarebio在组内，大致按激发波长的顺序排列（家族除外）。峰值激发和发射波长通常随探测器所处的环境而变化。所以需要自行确认所选染料的激发光谱和发射光谱。另外，下表显示的颜色只是一个非常粗略的近似值，并且不同显示设备会有一定色差！
ProbeEx (nm)Em (nm)MWNotesReactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAminocoumarin350445330Succinimidyl esterMethoxycoumarin360410317Succinimidyl esterCascade Blue(375);401423596HydrazidePacific Blue403455406MaleimidePacific Orange403551Lucifer yellow425528NBD466539294NBD-XR-Phycoerythrin (PE)480;565578240 kPE-Cy5 conjugates480;565;650670aka Cychrome, R670, Tri-Color, Quantum RedPE-Cy7 conjugates480;565;743767Red 613480;565613PE-Texas RedPerCP490675Peridinin chlorphyll proteinTruRed490,675695PerCP-Cy5.5 conjugateFluorX494520587(GE Healthcare)Fluorescein495519389FITC; pH sensitiveBODIPY-FL503512TRITC547572444TRITCX-Rhodamine570576548XRITCLissamine Rhodamine B570590Texas Red589615625Sulfonyl chlorideAllophycocyanin (APC)650660104 kAPC-Cy7 conjugates650;755767PharRedAlexa Fluor dyes [antibody conjugates] (Molecular Probes)Alexa Fluor 350343442410Alexa Fluor 4054014211028Alexa Fluor 430434540702Alexa Fluor 488499519643QY 0.92Alexa Fluor 500503525700Alexa Fluor 514517542714Alexa Fluor 532530555724QY 0.61Alexa Fluor 5465615721079QY 0.79Alexa Fluor 5555535681250QY 0.1Alexa Fluor 568579603792QY 0.69Alexa Fluor 594591618820QY 0.66Alexa Fluor 6106106291285Alexa Fluor 6336326481200Alexa Fluor 6476526681300QY 0.33Alexa Fluor 6606636911100Alexa Fluor 6806807021150Alexa Fluor 7006967191400Alexa Fluor 7507527761300Alexa Fluor 7907828041750Cy DyesCy2489506714QY 0.12Cy3(512);550570;(615)767QY 0.15Cy3B558572;(620)658QY 0.67Cy3.5581594;(640)1102QY 0.15Cy5(625);650670792QY 0.28Cy5.56756941128QY 0.23Cy7743767818QY 0.28DyLight dyes (Pierce)DyLight 350353432DyLight 405400420DyLight 488493518DyLight 549562576DyLight 594593618DyLight 633638658DyLight 649654673DyLight 680692712DyLight 750752778DyLight 800777794Nucleic acid probesHoechst 33342343483616AT-selectiveDAPI345455AT-selectiveHoechst 33258345478624AT-selectiveSYTOX Blue431480~400DNAChromomycin A3445575CG-selectiveMithramycin445575YOYO-14915091271Ethidium Bromide493620394Acridine Orange503530/640DNA/RNASYTOX Green504523~600DNATOTO-1, TO-PRO-1509533Vital stain, TOTO: Cyanine Dimer
TO-PRO: Cyanine MonomerThiazole Orange510530Propidium Iodide (PI)536617668.4LDS 751543;590712;607472DNA (543ex/712em), RNA (590ex/607em)7-AAD5466477-aminoactinomycin D, CG-selectiveSYTOX Orange547570~500DNATOTO-3, TO-PRO-3642661DRAQ5647681,697413(Biostatus) (usable excitation down to 488)Cell function probesIndo-1361/330490/4051010AM ester. Low/High Ca++,Fluo-3506526855AM ester. pH > 6DCFH5055355292'7'Dichorodihydrofluorescein, oxidized formDHR505534346Dihydrorhodamine 123, oxidized form,
light catalyzes oxidationSNARF548/579587/635pH 6/9Fluorescent ProteinsQYBRPSSourceY66H360442Y66F360508EBFP3804400.189AddgenemonomerEBFP23834480.5618AddgenemonomerAzurite3834470.5514monomerGFPuv385508T-Sapphire3995110.602625weak dimerTagBFP40245726k0.6333++EvrogenmonomerCerulean4334750.622736weak dimermCFP4334750.401364monomerECFP4344770.153CyPet4354770.511859weak dimerY66W436485dKeima-Red4406160.318MBLdimermKeima-Red4406200.243MBLmonomerTagCFP4584800.5729EvrogendimerAmCyan14584890.7529ClontechtetramermTFP1 (Teal)4624920.8554dimerS65A471504Midoriishi-Cyan4724950.925MBLdimerWild Type GFP396,47550826k0.7716S65C479507TurboGFP48250226 k0.5337EvrogendimerTagGFP48250527k0.5934++EvrogenmonomerTagGFP248350627k0.634++EvrogenmonomerAcGFP148451027k0.8227ClontechS65L484510Emerald4875090.68390.69Invitrogenweak dimerS65T488511EGFP48850726k0.6034174Addgeneweak dimerAzami-Green4925050.7441MBLtetramer (monomeric available)ZsGreen1493505105k0.9140ClontechtetramerDronpa-Green5035180.8581MBLphotoswitchableTagYFP50852427k0.6247EvrogenmonomerEYFP51452726k0.615160weak dimerTopaz5145270.6057monomerVenus5155280.575315weak dimermCitrine5165290.765949monomerYPet5175300.778049weak dimerTurboYFP52553826 k0.5356EvrogendimerPhiYFP52553726.8 k0.4052++Evrogenweak dimerPhiYFP-m52553726.8 k0.3948++EvrogenmonomerZsYellow15295390.6513ClontechtetramermBanana5405530.704ClontechmonomerKusabira-Orange5485590.6031MBLmonomermOrange5485620.69499monomermOrange25495650.6035ClontechmonomermKO5485590.6031122monomerTurboRFP55357426 k0.6762EvrogendimertdTomato5545810.699598Clontechtandem dimerDsRed-Express25545910.4215ClontechTagRFP55558427k0.4848EvrogenmonomerDsRed monomer557592~28k0.13.516ClontechmonomerDsRed2 ("RFP")563582~110k0.5524ClontechmStrawberry5745960.292615ClontechmonomerTurboFP60257460226 k0.3526EvrogendimerAsRed2576592~110k0.2113ClontechtetramermRFP1584607~30k0.25Tsien labmonomerJ-Red5846100.208.813dimermCherry5876100.221696ClontechmonomerHcRed1588618~52k0.030.6ClontechdimermKate258863326k0.4025+EvrogenmonomerKatushka (TurboFP635)58863526k0.3422++EvrogendimermKate (TagFP635)5886350.3015EvrogenmonomerTurboFP63558863526 k0.3422EvrogendimermPlum5906490.104.153ClontechmRaspberry5986250.1513Clontechmonomer; faster photobleach than mPlummNeptune6006500.2013Tsien LabmonomerE2-Crimson6116460.2329ClontechOther probesMonochlorobimane380461226Glutathione probeCalcein496517623pH > 5HyPer420/500516Hydrogen peroxide sensor
Legend:
Ex: Peak excitation wavelength (nm)Em: Peak emission wavelength (nm)MW: Molecular weightQY: Quantum yieldBR: Brightness; Extinction coefficient * Quantum yield / 1000PS: Photostability; time to 50% brightness (sec)